Moleculer Biology

OBJECTIVES AND SPECIFICATIONS:
– Ultra high throughput DNA sequencing with GS FLX of long and complex samples
– Large scale DNA sequencing studies, Amplicon library sequencing
– Transcriptome sequencing (“RNA-Seq”) to identify alternative splice-variants and discovery of novel transcripts in specific cell lines or tissues
– Gene Expression Analysis, Metagenomics for simultaneous sequencing of DNA from many different microbes extracted from biological or environmental samples
– De novo whole genome sequencing of previously un-sequenced species, or variant individuals for comparison to a reference sequence

Untitled-7ghh

AREAS OF IMPACT AND APPLICATIONS:
– Whole genome sequencing of all kinds of organisms for health, medicine, agriculture and energy fields

EQUIPMENT LIST
– Roche 454 DNA Sequencer / Genome Sequencer
– Agilent 2100 Bioanalyzer
– Roche The LightCycler® 480 Real-Time PCR System
– Roche Casy Cell Counter
– Roche NimbleGen MS 200 Microarray Scanner
– Beckman Coulter Biomek® 3000 Laboratory Automation Workstation Liquid Handling System
– LCMSMS (planned)
– Mastercycler® 384 Gradient Polymerase Chain Reaction
– SPR NaviTM 200 Multi-Parametric Surface Plasmon Resonance
– Nanodrop 2000c Spectrophotometer / Nanodrop 3300 Fluorospectrometer
– Allegra X15 Rultracentrifuge
– Thermo Scientific Ultra Low Temperature Freezers
– Q-Sense E1 Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)