Cell Culture

OBJECTIVES AND SPECIFICATIONS
– High-quality mammalian cell culturing with routine quality control (Mycoplasma, sterility, etc.)
– Engineering of genetically-modified human cells via viral infection
– Reprogramming of human somatic cells into induced pluripotent stem (iPS) cells
– Establishment of primary culture from patient-derived cells
– Continuous monitoring live cell proliferation, morphology and viability with label free assay

EQUIPMENT LIST
– Thermo Scientific humid CO2 Incubator
– Thermo Scientific Class II Biological Safety Cabinets
– Zeiss Primovert Inverted Microscope
– iCelligence/ xCelligence Real Time Cell Analysis
– Countess II FL Cell counter
– Eppendorf 5702/5424R RT and Refrigerated Centrifuge
– VWR BSS-3000 Liquid nitrogen (N2) cryostorage container
– Thermo Scientific -86 ULT Freezer