Molecular Biology / Microbiology

OBJECTIVES AND SPECIFICATIONS:
– Qualitative analysis of DNA and RNA molecules by PCR
– Direct sequencing studies of long, single-molecule genomic DNA, RNA, and Amplicons by NanoPore technology
– High-resolution separation and quantification of nucleic acids and proteins using microfluidic ‘Lab-on-a-chip’
– High-throughput screening of biological samples for gene expression and copy number variations using customiseable microarrays
– Food pathogen culture and analysis
– Development of nucleic acid aptamers as novel biomarkers

EQUIPMENT LIST
– Roche NimbleGen MS 200 Microarray Scanner
– Eppendorf Mastercycler® 384 Gradient PCR
– Agilent 2100 Bioanalyzer
– ONT MinION single-molecule sequencing device
– Nanodrop 2000c Spectrophotometer
– Nanodrop 3300 Fluorospectrometer
– GelDoc EZ gel visualization system
– Allegra X15 Refrigerated Centrifuge
– Heraus Laminar Flow Cabinets (BSL2)
– Thermo Scientific Ultra Low Temperature Freezers
– Bacterial incubator-shaker