Upconversion Nanoparticles
Upconversion nanoparticles (UCNP) represent a new generation of fluorescent labels with many attractive properties for life science and biomedical applications. These nanoscale particles have a remarkable ability to absorb low energy photons (NIR) and convert them into higher energy photons (Uv-Vis/ NIR). Distinguished from traditional fluorescence probes, as fluorescent labels, UC nanoparticles exhibit narrow emission peaks, low cytotoxicity, and higher detection sensitivity owing to the absence of autofluorescence background. As the UCNP nanoparticles excited with NIR radiation, deeper penetrability also could be accessed that will allow them to be utilized in such cancer imaging, high-sensitivity immunodiagnostics, digital pathology, deep tissue imaging, and others besides. With the help of conjugation chemistry, UCNPs surface can be functionalized with various biomolecules such as antibodies, oligonucleotides, and can be used as a label to detect biomarkers both in vitro and in vivo.
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